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KMID : 1128320110090020055
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Electrolytes & Blood Pressure
2011 Volume.9 No. 2 p.55 ~ p.62
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Effects of Dietary Salt Restriction on Puromycin Aminonu cleoside Nephrosis: Preliminary Data
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Jo Chor-Ho
Kim Su-A
Park Joon-Sung
Kim Gheun-Ho
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Abstract
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Proteinuria is a major promoter that induces tubulointerstitial injury in glomerulopathy. Dietary salt restriction may reduce proteinuria, although the mechanism is not clear. We investigated the effects of dietary salt restriction on rat kidneys in an animal model of glomerular proteinuria. Male SpragueDawley rats were used and divided into 3 groups: vehicletreated normalsalt controls, puromycin aminonucleoside (PA)treated normalsalt rats, and PAtreated lowsalt rats. PA was given at a dose of 150 mg/kg BW at time 0, followed by 50 mg/kg BW on days 28, 35, and 42. Sodiumdeficient rodent diet with and without additional NaCl (0.5%) were provided for normalsalt rats and lowsalt rats, respectively. On day 63, kidneys were harvested for histopathologic examination and immunohistochemistry. PA treatment produced overt proteinuria and renal damage. Dietary salt restriction insignificantly reduced proteinuria in PAtreated rats, and PAtreated lowsalt rats had lower urine output and lower creatinine clearance than vehicletreated normalsalt controls. When tubulointerstitial injury was semiquantitatively evaluated, it had a positive correlation with proteinuria. The tubulointerstitial injury score was significantly increased by PA treatment and relieved by lowsalt diet. ED1positive infiltrating cells and immunostaining for interstitial collagen III were significantly increased by PA treatment. These changes appeared to be less common in PAtreated lowsalt rats, although the differences in PAtreated normalsalt versus lowsalt rats did not reach statistical significance. Our results suggest that renal histopathology in PA nephrosis may potentially be improved by dietary salt restriction. Nonhemodynamic mechanisms induced by lowsodium diet might contribute to renoprotection.
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KEYWORD
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puromycin, diet, sodiumrestricted, proteinuria, kidney
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